https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15964 Wed 11 Apr 2018 15:36:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19642 Wed 11 Apr 2018 14:59:48 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14888 Wed 11 Apr 2018 14:03:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27445 Wed 11 Apr 2018 12:10:23 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44697 Thu 20 Oct 2022 09:51:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21485 Salmonella enteritidis) or saline on postnatal days (PNDs) 3 and 5 and later subjected to the formalin test at PNDs 7, 13, and 22. One hour after formalin injection, blood was collected to assess corticosterone responses. Transverse spinal cord slices were also prepared for whole-cell patch clamp recording from lumbar superficial dorsal horn neurons (SDH). Brains were obtained at PND 22 and the hypothalamus was isolated to measure glucocorticoid (GR) and mineralocorticoid receptor (MR) transcript expression using qRT-PCR. Behavioural analyses indicate that at PND 7, no significant differences were observed between saline- or LPS-challenged rats. At PND 13, LPS-challenged rats exhibited enhanced licking (p < .01), and at PND 22, increased flinching in response to formalin injection (p < .05). LPS-challenged rats also displayed increased plasma corticosterone at PND 7 and PND 22 (p < .001) but not at PND 13 following formalin administration. Furthermore, at PND 22 neonatal LPS exposure induced decreased levels of GR mRNA and increased levels of MR mRNA in the hypothalamus. The intrinsic properties of SDH neurons were similar at PND 7 and PND 13. However, at PND 22, ipsilateral SDH neurons in LPS-challenged rats had a lower input resistance compared to their saline-challenged counterparts (p < .05). These data suggest neonatal LPS exposure produces developmentally regulated changes in formalin-induced behavioural responses, corticosterone levels, and dorsal horn neuron properties following noxious stimulation later in life. These findings highlight the importance of immune activation during the neonatal period in shaping pain sensitivity later in life. This programming involves both spinal cord neurons and the HPA axis.]]> Sat 24 Mar 2018 08:03:35 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27631 Sat 24 Mar 2018 07:34:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28942 p < 0.05), as defined by a marked elevation in GFAP expression, within all 13 sites assessed within the ipsilateral (lesioned) hemisphere. We further observed significant increases in GFAP expression (p < 0.05) in 9 of the 13 contralesional sites examined. This work underscores that both the ipsilateral and contralesional hemispheres, at sites distal to the infarct, are very active many weeks after the initial occlusion, a finding that potentially has significant implications for understanding and improving the regeneration of the damaged brain.]]> Sat 24 Mar 2018 07:31:25 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22827 Sat 24 Mar 2018 07:16:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22108 Sat 24 Mar 2018 07:10:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42592 Fri 26 Aug 2022 09:36:19 AEST ]]>